P-316 Effect of nanoparticle-mediated delivery of miRNA 503 on the apoptosis of ovarian endometriosis cells

Abstract

Abstract Study question Is effective miRNA 503 conjugation with PLGA nanoparticle in apoptosis of human endometriosis cells? Summary answer Cell death increased with increasing the concentration of miRNA; thus, it can be suggested as a treatment for endometriosis. What is known already MicroRNAs (miRNAs) regulate post-transcriptional gene expression and play a crucial role in proliferation, differentiation, and apoptosis, which are key to the diagnosis of endometriosis.miRNA 503 causes apoptosis and is genetically inhibited in endometriosis patients. Therefore, in this study, we decided to introduce this miRNA into the endometriosis cell using PLGA nanoparticles in the laboratory Study design, size, duration The cells were divided into the five groups of control and four doses (25, 50, 75, and 100 µm) of miRNA 503/PLGA. The cells were then treated with the optimum dose obtained, and survival rates were compared with and without nanoparticles. The experiments were performed at 12, 24, 48, and 72 hours. Viability and apoptosis were evaluated by the MTT assay and Annexin Kits. Data were analyzed by one-way analysis of variance. Participants/materials, setting, methods In this study, endometriosis cells were isolated from women with severe endometriosis (DIE) and digested by the enzymatic method (40 µg/ml DNAase I and 300 µg/ml collagenase type 3). PLGA-based nanoparticles were synthesized and characterized. The size of sole PLGA nanoparticles was about 60 nm, and that of PLGA/miRNA was about 70 nm. Poly lactic-co-glycolic-based nanoparticles were used as vector carriers for miRNA 503 transfection in endometriosis cells. Main results and the role of chance Based on the MTT assay results, the optimal dose of miRNA 503 / PLGA was 75 µm, at which the viability of ECSCs was 52.6%±1.2 (P ≤0.05), and the optimal time was 48 h. The apoptotic rates of ECSCs treated with PLGA/miRNA503 (34.75±4.9%) were significantly higher than those of ECSCs treated with PLGA alone (3.35±2.58%) (P ≤0.05). Limitations, reasons for caution Cell mortality in vitro and animal survival after cell injection were among the limitations of the study. Wider implications of the findings miRNAs are involved in cell survival, proliferation, angiogenesis, and apoptosis, and therefore, they are effective in the pathogenesis of endometriosis . Viral and non-viral vectors are used for miRNAs transmission. Non-viral gene delivery vectors, such as inorganic nanoparticles and liposomes, have been used in recent years Trial registration number not applicable