P-305 Detection of GSTM1 polymorphism in women undergoing IVF protocols

Abstract

Abstract Study question Is GSTM1 polymorphism related to infertility and IVF parameters? Summary answer Possible correlation between the absence of GSTM1 gene and estradiol levels as well as the fertilized oocytes number, the number of COCs and their quality. What is known already Glutathione S-transferase (GST) M1 belongs to a family of detoxification enzymes. GSTM1 detoxifies reactive chemical species, by catalyzing their conjugation to glutathione. Deficiency in enzyme activity is caused by homozygous deletion of the GSTM1 gene. It is known that oxidative stress is a condition that leads to pathophysiological mechanisms related to female infertility Additionally several studies have addressed the possible correlation between female infertility and GSTM1 polymorphisms Study design, size, duration One hundred and seventy four samples were collected and divided into 2 groups. The study group was consisted of blood samples from 125 women classified as infertile according to WHO. Blood samples from 49 women classified as fertile with at least one successful pregnancy and nomiscarriages were used for the control group Participants/materials, setting, methods For all samples, genetic material(DNA) was extracted. Polymerase chain reaction with specific GSTM1 gene primers followed by agarose electrophoresis was applied to detect the presence of polymorphism. Main results and the role of chance Our study showed a significant difference between the presence of GSTM1 gene in the control group when compared with the study group. The results showed for a subgroup of the infertile women,(n = 54), that the estradiol levels were significantly higher, in women who had the GSTM1 gene compared to those who lacked the gene. The fertilized oocytes as well as the number of COCs were significantly higher in women in which the GSTM1 was detected, compared to the women in which the GSTM1 gene was not detected. Limitations, reasons for caution Detailed data from all infertile women could further confirm our results. Wider implications of the findings The results of the current study may be used for further research related to female infertility and oxidative stress and may clarify the possible interactions between the microenvironment of oocytes and the free radicals. Additionally GSTM1 may be a useful biomarker for predicting IVF results. Trial registration number not applicable