P-247 - Anti-tumor properties of the redox-active compound Parvifloron D in breast cancer cells

Abstract

Medicinal plants have been a prolific source of new bioactive compounds with anticancer activity. Parvifloron D (ParvD) is an abietane diterpenoid isolated from Plectranthus ecklonii acetonic maceration extract. ParvD was previously shown to have a strong reducing capacity (DPPH) and to protect DNA from oxidative breakage (plasmid strand break analysis). In addition, ParvD has shown cytotoxic and pro-apoptotic effects in leukemia and melanoma models. Herein, the anticancer effect of ParvD was evaluated in MDA-MB-231 human breast cancer cells. ParvD (0.1–10 μM) decreased cell viability in a concentration-dependent manner. Treatment with 5 μM ParvD significantly increased the percentage of apoptotic nuclei. Cell exposure to 3 μM ParvD increased the sub-G1 population. Treatment with ParvD (1 μM) had no effect on cell-substrate attachment when cell detachment was induced with EDTA. The effect of ParvD on cell chemotaxis and invasion was evaluated by a transwell assay. ParvD (1 μM) significantly reduced cell migration and invasion, which are determinant processes for the formation of metastases. In summary, the redox-active natural compound ParvD showed interesting anticancer properties and should be further studied towards a potential therapeutic application.