P 205 - Effects of metabolites derived from Hibiscus sabdariffa on high glucose-induced oxidative stress and inflammation in hypertrophied 3T3-L1 adipocytes

Abstract

Recent evidences indicate that polyphenols from Hibiscus sabdariffa L. (HS) may become an alternative against metabolic disturbances associated to obesity. Our findings strongly support that quercetin derivatives from HS are absorbed and metabolized in vivo and suggests that these metabolites are the major blood compounds accounting for these effects. The purpose of this work was to determine the effect of several quercetin derivatives (quercetin, quercetin-3-glucuronide and quercetin-3-glucoside) compared with others of recognized capacity to reduce oxidative stress and inflammation in a high-glucose hypertrophied adipocyte model. The results indicate that HS and quercetin derivatives reduce glucolipotoxicity-induced oxidative stress in 3T3-L1 adipocytes and INS832/13 β-cells. Furthermore, among all the polyphenolic compounds tested, quercetin derivatives were the more active compounds reducing ROS generation in hypertrophied 3T3-L1 adipocytes. These flavonoids also modulated pro-inflammatory adipokine secretion and reduced the expression of MCP-1 cytokine at transcriptional level in the hypertrophied cell model. In conclusion, these data suggest that quercetin derivatives seem to be the main responsible compounds of the antioxidant and anti-inflammatory activities of HS extract.