P.19.8 The effect of water-soluble fullerene with different number of hydroxyl groups in muscle regeneration process of experimental murine skeletal muscle

Abstract

Muscular dystrophy is a hereditary disease, which cause severe muscle weakness and atrophy in clinically, and skeletal muscle degeneration and necrosis in pathology. In recent years, reducing oxidative stress is considered as one of the new treatment strategy in muscular dystrophy. Fullerene is a good candidate to detoxify and absorb free radical. We have already revealed that fullerene has a function to promote the regeneration of skeletal muscle. To evaluate the effectiveness of different type of water-soluble fullerenes, we chronologically evaluate the histology in mice tibial muscles during a cycle of regeneration induced by cardiotoxin injection. We used three types of water-soluble fullerene, 44 hydroxyl fullerene, fullerene with PVP, and fullerene with hydrophilic cyclodextrin. Tibial muscles of C57BL10 mice (4–5 weeks old) were injected with cardiotoxin with 3 different types of fullerene or without fullerene. The injected muscles were removed and stained H& E on 1, 3, 5, 7, 14, and 28 days after the injection. Western-blotting was performed to evaluate expression of muscle proteins, such as dystrophin, desmin, and nNOS. Average diameter of regenerate muscles 28 days after injection, fullerene group was bigger than those of cardiotoxin group. There was no difference of average diameter with 3 types of fullerene. Muscle protein expression in co-administered fullerene group, dystrophin, desmin, and nNOS was observed in the earlier stage by Western blot than those of cardiotoxin group. During the experiment, there is no significant and critical change was observed. We revealed that 44 hydroxyl fullerene, fullerene with PVP, and fullerene with hydrophilic cyclodextrin has the same function to promote the regeneration of skeletal muscle in this experiment. We believe that water-soluble fullerene reduce oxidative stress and can be applied in the treatment of muscular dystrophy.