P 184 - Functional state and morphology of mesenchymal stem cells after oxidative stress

Abstract

Mesenchymal stem cells (MSCs) are an important member of the stem cell family and can be found in most postnatal organs and tissues. Due to multilineage differentiation potential, broad range of bioactive molecules secretion and other properties, these cells are being studied for their potential use in different modes of therapy. But a number of reports point to oxidative stress have been hypothesized to lead to loss of transplanted MSCs from the injured sites. Therefore, the understanding of the contributions of oxidative stress to MSC biology is required. The purpose of this study was to investigate the functional state of adipose-derived MSCs after oxidative stress. The cells treated with hydrogen peroxide (50, 200, 500 mkM) for 6 h were washed and further incubated in fresh medium for 4 days. We found cell viability was less 5% at 500 mkM H2O2 and exclude this concentration from analysis. In other samples a part of living cells was high (more 85%), but cell grow rate was significantly decreased after oxidative stress. Also the treated MSCs were enlarged in size and displayed more granular cytoplasm. Intracellular reactive oxygen species (ROS) level, activity of lysosome and mitochondrion compartments were increased.