P-181 The impact of high proportion of immature oocyte in a cohort on the reproductive outcome following icsi

Abstract

Abstract Study question Whether high proportion of immature oocyte in a cohort impacts outcome of sibling mature oocyte in ICSI cycles. Summary answer Study demonstrate that if immature oocyte retrieved was more than 50% in a cycle then it reduces reproductive outcome with the remaining mature sibling oocytes. What is known already The selection of competent oocyte is crucial to assisted reproductive technique procedure outcome. In ICSI procedure where only metaphase II oocytes (MII) are injected correlates with number of MII oocytes available, embryo development and pregnancy outcome. Percentage of immature oocyte {Metaphase I (MI) and Germinal Vessicle (GV) } retrieved in a cycle is always a concern for stimulation protocol, patient response which affects IVF laboratory outcome. Study design, size, duration A prospective study was conducted from 1st January 2020 to 1st December 2022 in the Department of Reproductive medicine at a tertiary infertility centre in India. Participants/materials, setting, methods Patients were divided into 3 groups on the basis of percentage of immature oocyte retrieved in a single cycle. Group A (n = 100), where percentage of immature oocyte (MI and GV) was between 0 to 20%, while Group B (n = 60) where percentage of immature oocyte was between 21 to 50% and Group C (n = 20) where percentage of immature oocyte was between 51 to 100%. Main results and the role of chance This study included all normal responder patients and cycles using in-vitro fertilisation (IVF) and poor responders were excluded. All blastocyst formed were vitrified and transferred in frozen embryo replacement cycle. All the 3 groups were compared on the basis of fertilisation rate, blastocyst formation rate, clinical pregnancy rate and miscarriage rate. Group A and group B had no significant difference in fertilisation rate (82% vs. 78%, p > 0.5), blastocyst formation rate (60% vs. 58%, p > 0.5), clinical pregnancy rate (58% vs. 55%, p > 0.5) and miscarriage rate (12% vs. 11%, p > 0.5). While significant reduction was observed in group C ( > 50% MI & GV oocyte) in fertilisation rate (40%, p < 0.1), blastocyst formation rate (28%, p < 0.1), clinical pregnancy rate (15%, p < 0.1). Limitations, reasons for caution Larger randomised control studies are needed to strengthen these results. Wider implications of the findings Percentage of immature oocyte can help in predicting the success of assisted reproductive technique. This will help in counselling patient about outcome. Trial registration number NA