P 129 - Alteration of phospholipidome profile in the heart of an animal model of acute myocardial infarction

Abstract

Acute myocardial infarction (AMI) is one of the main causes of mortality and health care cost in the world. Development of valuable clinical tools can help in diagnosis in the early stage of disease or predicting recovery. The pathogenesis of AMI is multifactorial and lipids can have an active role in this pathology and be good candidates as biomarkers of initial stages of the disease and extent of tissue damage. Changes in phospholipidome profile were observed in cardiomyoblasts as model of AMI. However, lipidomics studies in AMI are scarce. In this work, a mass spectrometry-based lipidomics approach was used to evaluate changes in phospholipid profile in heart of murine models under ischemia, ischemia-reperfusion and starvation conditions. Differences in fatty acids and phospholipid profiles, related to the course of AMI were observed. An increase of C16:0, C18:0, C20:4 and C22:6, and a decrease of C18:2 were observed in ischemia-reperfusion and starvation. An increment of PL bearing FA 22:6, such as PC(18:0/22:6), PE(16:0/22:6), and PE(18:0/22:6), occurred in the same conditions. These PL can be precursors of anti-inflammatory lipids (e.g. resolvins) involved in AMI recovery. Therefore, lipidomics profiling by mass spectrometry can offer valuable information to understand AMI that can be exploited for diagnosis and prognosis.