Abstract
Introduction: The extent of cellular radiation damage is dependent on the stage of the cell cycle and new methods to determine this are frequently being developed. Here we used bromodeoxyuridine (BrdU) that is incorporated into newly synthesized DNA and is detected using an immunofluorescent anti-body. This method aims to quantify the fraction of cells in S-phase as BrdU acts as a thymidine analogue. The advantages of using BrdU are that it allows for the identification and analysis of actively cycling cell fractions and also permits the determination of cell cycle kinetics. The aim of this study was to compare S-phase fractions observed using flow cytometry with microscopy methods.
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