P.1.g.098 Allomargaritarine as a basis for the creation of mitochondrial targeted potential neuroprotectors

Abstract

Group II (SB-269970): SB-269970 1mg/kbw; Group III (NAN190): NAN-190 1mg/kbw. Working memory was assessed using the radial-arm maze test. The design of this device ensures that after checking for food at the end of each arm the animal is forced to return to the central platform before making another choice. The following measures were recorded: the number of entering an arm containing food, but previously entered (working memory errors); the number of entering an arm that was not baited (reference memory errors); time taken to consume all five baits and the number of arms entered until a repeat entry was made (entries to repeat). Facilitation of efficient search behavior required an increase in the number of novel arms entered in the first eight choices, rather than simply an increase in total arms entered during the session. The data were presented as mean ± standard deviation and significance was tested by SPSS Statistics for Windows version 17.0 and ANOVA method. Experimental protocol was implemented following the recommendations of the ‘Gr. T. Popa’ University Committee for Research and Ethical Issues guidelines for handling and use of experimental animals, according to the ethical standards of the European Community. Results: The administration of selective serotonin 5-HT7 receptor antagonist SCH-23390 resulted in a significant decreasing (p< 0.05) of the number of working memory errors (relevant for short-time memory evaluation) and of average time taken to consume all five baits (p< 0.05), but did not influence the number of reference memory errors (relevant for long-time memory quantification) compared to control group. The treatment with the selective 5-HT1 receptor antagonist NAN-190 was not associated with a significant modification of the novel choices arm entries comparing with saline group, in radial arm maze in rats. Conclusions: The results revealed that SB-269970 produced a short-time memory retention enhancement and an improvement of discriminative spatial learning, thus corresponds to favorable effects on short-time memory, without affecting long-time memory of lab animals. In our experimental conditions, 5-HT1 receptor antagonist NAN-190 intraperitoneal injection did not significantly influence the cognitive functions in rats.