P.1.g.083 Effects of apocynin on retrograde memory dysfunction and glial response induced by transient global cerebral ischemia in rats

Abstract

50mM for U251 and U87 cell lines and 100 mM for C6 cell line). When applied in its IC50 concentration, desloratadine increased production of ROS in U251 (1.4 times, compared to the control) as well as in other cell lines. It was also noted an increased caspase activity which implies to caspase-dependent apoptosis. Using double staining with Annexin V (An) and propidium iodide (PI), other apoptotic marker (extravasation of phosphatidyl serine) was noted after the tretment (33% of apoptotic cells in comparison to control that had 1.7%). Cell cycle analysis showed higher percent of apoptotic cells in sub G0 phase in C6 cell line (increased FL2 fluorescence). Increased presence of the acid cytoplasmic vesicles (quantified as an increase of the orange-FL3 vs. greenFL1 fluorescence, compared to the control) indicated a presence of autophagy in all investigated cell lines. Immunoblot method revealed the increased expression of LC3II after 18 h and 24 h of treatment, in dose-dependent manner. Conclusion: Desloratadine exerts cytotoxic effects on exammined cell lines, inducing apoptosis and autophagy. On view of the results showen above, we can conclude that its cytotoxic effect is not cell type speciffic.