P.097 Metabolomic and lipidomic profiling of high and low grade gliomas - a matched serum and tissue clinical study

Abstract

Background: It is well understood that gliomas require vast supply of energy to proliferate, invade and spread. We wished to identify novel biomarkers by comparing normal brain and plasma to high and low grade gliomas using newer techniques in laser ionization mass spectroscopy - precision metabolomics and lipidomics. Methods: Single center IRB approved tissue bank of “normal” brain and plasma (n=6) and IDH wild-type GBM tissue and plamsa (n=29), IDH mutant GBM tissue and plamsa (n=6), Low grade glioma (n=4) tissue and plamsa were analyzed for over 2000 endogenous metbolites and complex lipids. Unbiased clustering and Random Forest plots and pathway analysis were performed with appropriate statsitical analysis (significance p < 0.05). Results: IDH mutant GBM had higher levels of 2-HG, however, plasma 2-HG did not reflect IDH genotype. Changes in glucose and fatty acid utilization were observed in IDH WT and mutant gliomas compared to normal brain tissue. Lipidomics of plasma and tissue of normal and gliomas did not reveal a biomarker reaching statistical significance. Conclusions: We will continue to investigate if plasma and tissue biomarkers including hypotaurine, methionine, branched chain amino acid catabolites and pregnonolone can be used to predict tumor progression, response to treatment and clinical outcomes.