P-016 Effects of the different partial deletions in the AZFc locus of Y-chromosome on the ICSI outcome of severe oligospermia patients

Abstract

Abstract Study question To investigate the effects of different partial deletions in AZFc locus of Y-chromosome on the clinical outcome of severe oligospermia patients by intracytoplasmic single sperm injection (ICSI) Summary answer B2/b3 and b2/b4 deletion in the AZFc locus of Y chromosome have no significant effect on pregnancy outcome in patients with severe oligospermia undergoing ICSI. What is known already Thanks to the development of high-throughput sequencing for Y chromosome microdeletion, AZFc deletions can be accurately divided into b2/b4, gr/gr, b2/b3 and b1/b3 types. However, there is no relevant research on the effect of different partial deletion types of AZFc on the outcome of assisted reproduction. Study design, size, duration According to whether carrying AZFc microdeletions or not, the patients were divided into AZFc-deletion group and control group. And AZFc-deletion group was divided into 3 subgroups by the types of partial deletion. Participants/materials, setting, methods A retrospective analysis was conducted on the patients undergoing high-throughput sequencing for Y chromosome microdeletion screening and ICSI treatment in our hospital from December 2017 to February 2019. Main results and the role of chance The AZFc b2/b3 deletion group had no significant difference in D3 availability, high-quality embryo rate, blastocyst formation rate, implantation rate, clinical pregnancy rate and live birth rate, and the control group (P > 0.05). The rate of high-quality embryos in patients with the b2/b4 deletion was lower than that of the control group (23.2% vs. 34.3%, P = 0.004), but there was no statistical difference in other indicators. Limitations, reasons for caution In this study, we included only patients who underwent ICSI with ejaculate sperm for analysis, but not patients who had successfully obtained sperm through surgery. Wider implications of the findings This study helps clinicians provide more detailed and comprehensive infertility risk answers during consultations with such patients to reduce the mental stress of patients. Trial registration number not applicable