Ozone therapy and periodontal disease: aclinical-microbiological study

Abstract

Event Abstract Back to Event Ozone therapy and periodontal disease: aclinical-microbiological study Marta Dani1*, Lorenzo Azzi1, Alessandro D'Aiuto1, Fabio Croveri1, Vittorio Maurino1 and Lucia Tettamanti1 1 University of Insubria, Department of Medicine and Surgery, Italy Aim. The periodontal disease hits the 60-70% of population in Italy; the 10-14% of them are affected by a severe form of periodontal disease. The people affected by periodontal disease have higher risk to develop atherosclerosis, cardiovascular disease, cerebrovascular disease, diabetes mellitus and pregnancy complications. The ozone therapy is widely used in medical world; ozone plays several actions such as anti-microbial, anti-inflammatory, analgesic, immune-stimulating, anti-hypoxic, bio-energetic and bio-synthetic. The aim of this work is to detect differences between a group treated with causal therapy and ozone therapy, compared with a group treated with causal therapy only. Materials and Methods. The population sample analyzed was composed by 14 individuals affected by chronic periodontal disease, 7 men and 7 women, 5 smokers; the age of the sample was between 35 and 78 years old, the middle age was 55,4 years old. For each individual one arch was treated by causal therapy and the other arch was treated by ozone therapy plus causal therapy. The arch which was treated by ozone therapy has been chosen by random criteria. The ozone therapy consisted in the application of a galenic compound based on ozone: OZOSAN® - Gel Complex (Sanipam S.r.l.). At T0 for each individual a periodontal card was filled out and microbiological samples from the periodontal pockets were collected. The same data have been also collected at T1, 4 months after the end of the treatment. The microbiological samples were analyzed by PCR Real Time technology. The inhalation is the main problem of ozone; the ozonized gel was applied to the patient by a silicon mask, with the aim of avoid the inhalation. Results. The ozone therapy has produced a moderate improvement in probing depth. For what concern the bacterial count we underlined the significant reduction of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Fusocacterium nucleatum. Discussion. We can adfirm that the ozone therapy improves the traditional causal therapy. Ozone does not produce a significant clinical improvement, measurable with depth probing. The main action of ozone is reducing significantly the bacteria of the red complex and some bacteria of the orange complex. The bacteria of the red and orange complex are the major responsible of periodontal disease; they are anaerobic bacteria, so they can’t survive in the aerobic environment, created by ozone therapy. The bacterial load of Campylobacter rectus doesn’t decrease after ozone treatment. Campylobacter rectus is one of the early colonizers of periodontal pocket; if other bacteria are eliminated by ozone, he can quickly recolonize the periodontal pockets. Therefore the ozone therapy in addition to traditional causal therapy improves the periodontal disease, mostly for what concern the microbiota.