Ozone exposure differentially affects oxidative stress parameters in distinct Phaseolus vulgaris L. varieties

Abstract

Different oxidative stress parameters of four Brazilian varieties of Phaseolus vulgaris exposed to toxic O3 concentrations were compared with the well-established bioindicator variety Pinto 111. Analysis of catalase (CAT) activity, lipoperoxidation and non-enzymatic antioxidant levels demonstrated that the varieties presented different susceptibilities to O3 toxicity. Results indicated that Fepagro 26 and Guapo Brilhante increased the levels of lipoperoxidation in response to O3 while catalase activity was not changed. On the opposite Iraí, Macotaço and Pinto 111 presented different pattern of lipoperoxidation and CAT activation indicating a relationship between CAT activation and resistance to lipoperoxidation. Analysis of the total non-enzymatic antioxidant defense levels demonstrated that although O3 exposure decreased the non-enzymatic antioxidant defense in Iraí, lipoperoxidation levels was not enhanced in this variety as observed with Fepagro 26. Our results suggest that analysis of the oxidative stress parameters of varieties with known variations of susceptibility to O3 may provide more precise protocols for early detection of O3 toxicity.