Abstract
Objective This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Methods This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson's trichrome staining and microscopic evaluation. Results There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of p=0.001(p < 0.05), resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann–Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of p=0.009(p < 0.05). Conclusions Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.
Highlights
Full-thickness wound is an injury that extends from the epidermis, dermis, fat layer, fascia, even to the bone [1]
The platelet-rich-fibrin matrix and macrophages are gradually replaced by granulation tissue, which is composed of clusters of fibroblasts, macrophages, and endothelial cells that form the extracellular matrix and neovascularization
From the Kruskal–Wallis test followed by the post hoc analysis using the Mann–Whitney test, it was observed that the number of fibroblasts in full-thickness skin defects tended to increase in wound care using 1800 mg ozonated Aloe vera oil compared to wound care receiving only Aloe vera oil (K1+ and P3) on day 3 by 110.6 (39.2–157.2) μm2 and day 7 by 242.2 (168.8–265) μm2
Summary
Ahsanu Taqwim Hidayat ,1 Muhamad Thohar Arifin ,1,2 Muhammad Nur ,3 Muflihatul Muniroh ,1,4 and Neni Susilaningsih 1,5. Is study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Is was an experimental research method using control groups and treatment groups with a posttest only control group design. Ere was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of p 0.001 (p < 0.05), resulting in a significant difference in wound size reduction between the groups. Ozonated Aloe vera oil is effective in increasing the healing response of fullthickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats
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