Abstract

Oxyresveratrol has been proven effective in inhibiting adipogenesis in a 3T3-L1 cell model. We investigated the preventive effect of oxyresveratrol supplementation on obesity development in high-fat diet-fed mice. Male C57bl/6 mice were randomly subjected to control (5% fat by weight, LF), high-fat (30% fat by weight, HF), and high-fat supplemented with 0.25% and 0.5% oxyresveratrol (OXY1 and OXY2, respectively) diet groups for eight weeks. Oxyresveratrol supplementation effectively alleviated obesity-associated symptoms such as insulin resistance, hyperglycemia, and hepatic steatosis in high-fat diet-fed mice. Compared to the high-fat diet group, oxyresveratrol supplementation suppressed expression of glucose-6-phosphatase, sterol regulatory element-binding proteins 1, fatty acid synthase and CCAAT/Enhancer-binding proteins α, and elevated AMP-activated protein kinase (α2-catalytic subunit) level in liver, upregulated insulin-dependent glucose transporter type 4 level in adipose tissue, and increased expression of insulin receptor substrate 1, insulin-dependent glucose transporter type 4, AMP-activated protein kinase α, peroxisome proliferator-activated receptor γ coactivator-1α, and sirtuin 1 in muscle to regulate lipid and glucose homeostasis in these tissues. This study demonstrated that oxyresveratrol supplementation effectively ameliorated obesity-associated symptoms in high-fat diet-fed mice, presumably attributed to mediating critical regulators involved in lipid and glucose homeostasis in liver, visceral fat, and muscle.

Highlights

  • Over the years, the prevalence of obesity has been increasing worldwide with the obese population more than doubling since 1980

  • Comparing the two OXY supplemented groups, both body weight gain and energy efficiency were significantly lower in the OXY2 group, while energy intakes were not significantly different (Figure 2, Table 2, p < 0.05)

  • Our previous in vitro study determined that OXY possesses anti-adipogenic property in 3T3-L1 cells by regulating some key transcriptional factors, as well as inducing cell cycle arrest through modulation of specific cell cycle regulatory molecules [17], which encouraged us to study the potential effect of OXY in regulating obesity in vivo

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Summary

Introduction

The prevalence of obesity has been increasing worldwide with the obese population more than doubling since 1980. More than 1.9 billion adults were overweight or obese in 2014. In 2014, 41 million children under the age of 5 were reported to suffer from overweight or obesity [1]. More and more adults as well as children suffer from abnormal or excessive body fat with a variety of comorbidities, such as hypertension, dyslipidemia, type 2 diabetes, cardiovascular disease, stroke, sleep apnea, knee osteoarthritis, and certain cancers [2,3]. To prevent or heal obesity with the supplementation of phenolic compounds have generated intense interest in recent years. Supplementation of resveratrol is capable of relieving the harmful effects induced by a high-calorie diet such as reducing the rodents body weight gain, adipose tissue depots, plasma triglycerides, and increasing their survival and motor function [5]

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