Abstract
Oxygenated unsaturated fatty acids, known as oxylipins, are signaling molecules commonly used for cell-to-cell communication in eukaryotes. However, a role for oxylipins in mediating communication in prokaryotes has not previously been described. Bacteria mainly communicate via quorum sensing, which involves the production and detection of diverse small molecules termed autoinducers. Here we show that oleic acid-derived oxylipins produced by Pseudomonas aeruginosa function as autoinducers of a novel quorum sensing system. We found that this system controls the cell density-dependent expression of a gene subset independently of the quorum sensing systems thus far described in this bacterium. We identified a LysR-type transcriptional regulator as the primary receptor of the oxylipin signal. The discovery of this oxylipin-dependent quorum sensing system reveals that prokaryote-derived oxylipins also mediate cell-to-cell communication in bacteria.
Highlights
Oxygenated unsaturated fatty acids, known as oxylipins, are signaling molecules commonly used for cell-to-cell communication in eukaryotes
We recently reported that the oxylipins (10S)-hydroxy-(8E)octadecenoic acid (10-HOME) and 7S,10S-dihydroxy-(8E)-octadecenoic acid (7,10-DiHOME) generated by the diol synthase activity are involved in several biological processes, such as motility, biofilm formation and virulence in P. aeruginosa[16]
We found that addition of oleic acid (OA) to the culture was required to isolate a periplasmic fraction of P. aeruginosa displaying diol synthase activity in vitro (Fig. 1a)
Summary
Oxygenated unsaturated fatty acids, known as oxylipins, are signaling molecules commonly used for cell-to-cell communication in eukaryotes. We tested the ability of 10-HOME and 7,10-DiHOME oxylipins, purified from a PAO1 culture supernatant, to induce the β-gal activity in ΔDS (pDSplacZ). To determine the level of the diol synthase operon expression in relation to the cell density, the kinetics of β-gal activity in PAO1 (pDSp-lacZ) was monitored.
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