Oxygen Transport by Erythrocyte/Hemoglobin Solution Mixtures in anin VitroCapillary as a Model of Hemoglobin-Based Oxygen Carrier Performance

Abstract

Oxygen transport behavior of erythrocyte/extracellular hemoglobin mixtures flowing in microvessels was studied as a model of hemoglobin-based oxygen carrier (HBOC) performance. An experimentalin vitro25-μm-diameter capillary model was used to provide detailed oxygen flux measurements for hemoglobin solutions, erythrocyte suspensions, and erythrocyte/hemoglobin solution mixtures. The experimental apparatus includes computerized data acquisition and control coupled to a dual wavelength microspectrophotomer. This apparatus had been previously validated by good agreement of experimental measurements with predictive mathematical models of oxygen transport for either erythrocyte suspensions or hemoglobin solutions. The experimental methodology was extended to measurement of oxygen transport in erythrocyte/hemoglobin solutions. The hemoglobin solutions consisted of either purified or gluteraldehyde polymerized bovine hemoglobin. Dose–response plots were generated by varying the extracellular to intracellular hemoglobin ratio while holding the overall hemoglobin concentration constant. Measurements were also made on unmixed erythrocyte suspensions and hemoglobin solutions to generate limiting cases for comparison. Direct comparison of experimental results showed that both types of hemoglobin solutions were substantially more efficient than erythrocyte suspension in uptake and release of oxygen. Increased extracellular hemoglobin concentration increased oxygen transport efficiency for both uptake and release, even when total hemoglobin concentration was held constant. When only 10% of the total hemoglobin was extracellular, approximately half of the increased efficiency of pure hemoglobin solutions was reached. When 50% of the total hemoglobin was extracellular, the increased efficiency was virtually equal to that of pure hemoglobin solutions.