Oxygen-plasma treatment-induced surface engineering of biomimetic polyurethane nanofibrous scaffolds for gelatin-heparin immobilization

Abstract

AbstractPolyurethane (PU) has been extensively used in vascular tissue engineering due to its outstanding mechanical performance and blood compatibility behavior. Here, biomimetic PU-based scaffolds were prepared using an electrospinning technique and gelatin-heparin was introduced as a surface modifier after oxygen plasma treatment to improve cell attachment and release an anticoagulation agent. Morphology, Fourier transform infrared (FTIR) spectroscopy, compression strength, swelling and biodegradation ratio, drug release level and cellular interactions were evaluated. According to the scanning electron microscopy (SEM) micrographs, gelatin-heparin immobilized PU nanofibers exhibited a smooth surface and a bead free structure that nanofibers distributed in the range of 300–1000 nm. The mechanical strength of constructs, swelling and biodegradation ratio, and drug release level illustrated higher values for oxygen plasma-treated samples compared with bilayered scaffolds. Cellular adhesion and biocompatibility ameliorated after plasma treatment. All the mentioned findings indicated the initial physicomechanical and biological potential of biomimetic PU-based fibers in the improvements of vascular scaffolds.