Oxygen Free Radical Scavenger PtPd@PDA as a Dual-Mode Quencher of Electrochemiluminescence Immunosensor for the Detection of AFB1.

Abstract

Here, a dual-mode quenched electrochemiluminescence (ECL) immunosensor based on PtPd@PDA was proposed. Among them, nitrogen-doped hydrazide conjugated carbon dots (NHCDs), as an ECL emitter and a donor of resonance energy transfer, were quenched by PtPd@PDA (receptor). At the same time, PDA in PtPd@PDA, as an oxygen radical scavenger, completed the further quenching of the ECL signal by consuming O2•- generated by the decomposition of co-reactant H2O2. The dual-mode quenching from the above two channels was achieved. In addition, compared with the traditional carbon quantum dots, NHCDs as ECL emitters had lower excitation potential. Moreover, a large number of amino groups provided by aminated MWCNTs could capture more antibodies while connecting with NHCDs. Under the optimum experimental conditions, taking aflatoxin B1 as the target, the proposed sensor with good specificity, stability, and reproducibility had good linearity when the concentration of AFB1 was 0.01-100 ng/mL, with the detection limit of 2.63 pg/mL (S/N = 3). This strategy provided more possibilities for the application of dopamine metal nanocomposites in electrochemiluminescence analysis and offered a new approach to detect AFB1.