Abstract
1. 1. Blood from capture-stressed striped marlin cannot be fully saturated with oxygen in the presence of lactic acid because of a substantial Root effect. Titration of blood to pH 7.6 at 25°C gave an O 2 affinity constant, P 50 = 17 mmHg. The P 50 was highly sensitive to pH showing a Bohr factor Φ = Δ log P 50/ ΔpH = −0.74. 2. 2. Blood from capture-stressed raako sharks did not show either a Root effect or a significant Bohr effect (Δ log P 50 ΔpH = +0.16 ). The O 2-affinity constant, P 50, was 10.6 mmHg at pH 7.6 and 25°C. 3. 3. Hematological measurements following capture gave hematocrit values of 24.2±8.1% and 32.3 ± 10.8% for marlin and mako respectively, and high concentrations of blood lactate in both species (15–16mmol 1 −). 4. 4. The O 2 transport properties of the blood are discussed in relation to oxygen demand and capture stress.
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