Abstract
Background Nanobubbles NBs are small gas particles with diameters less than nm and have unique properties such as long retention times and high gas content in liquids Oxygen and air NBs are reportedly bioactive promoting growth in animals however there are few reports on the mechanisms governing such effects Accordingly this study investigated the effects of oxygen and air NBs on the proliferation of dental follicle stem cells DFSCs in vitro Methods DFSCs were obtained from an impacted supernumerary tooth The cells were cultured with Dulbecco rsquo s modified Eagle rsquo s medium containing oxygen or air NBs water for or days Each cell number was measured by a colorimetric assay using CCK nbsp Results and discussion Oxygen and air NBs significantly inhibited cell proliferation in a dose dependent manner However there was no significant difference between oxygen and air NBs media High oxygen content may have inhibited cell proliferation nbsp
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