Oxigenoterapia hiperbárica na cicatrização de feridas em camundongos

B.P Amaral,M.A Inkelmann,J.S Pasini,Á.J.C Silva,A Krause,D.C.M Müller

doi:10.1590/1678-4162-11638

Abstract

ABSTRACT We investigated the local and systemic effects of hyperbaric oxygen therapy in BALB/C mice, exposed to two different exposure times, under 2.4 atmosphere (ATM). Fifteen animals were divided into three groups (GI, GII and Control) and underwent a surgical excision of a skin fragment of approximately one square centimeter of the dorsal region. The wounds were treated and monitored for 21 days. In the control group, the wound was cleaned once a day with sterile 0.9% NaCl solution. GI and GII mice were submitted to daily hyperbaric oxygen therapy of 30 or 60minutes sessions, respectively. The wounds were photographed every three days and their surfaces were analyzed by an image analyzer. At 21 days, all animals were euthanatized for histopathological analysis of the skin, lungs and liver in order to identify eventual alterations in wound healing or in the analyzed organs. Animals belonging to GI showed a faster skin wound healing in comparison to the other groups. Animals from GII, however, showed a delayed wound healing process and exhibited lung and microcirculatory alterations. These findings allow us to conclude that the exposure time to the oxygen in hyperbaric environment is crucial and can help or disturb skin wound healing or even be deleterious to other organs.

Highlights

Clinical management of cutaneous wounds has great relevance in veterinary medicine, especially in extensive lesions and in patients with healing disorders, such as diabetics (Vermeulen et al, 2005; Waldron and Zimmerman-Pope, 2007)
We investigated the local and systemic effects of hyperbaric oxygen therapy in BALB/C mice, exposed to two different exposure times, under 2.4 atmosphere (ATM)
Along with contributing to the reversion of hypoxia, Hyperbaric oxygen therapy (HBOT) stimulates the formation of the collagen matrix, which is essential for angiogenesis and healing (Kiumehr et al, 2005; Zhang et al, 2007)

Summary

INTRODUCTION

Clinical management of cutaneous wounds has great relevance in veterinary medicine, especially in extensive lesions and in patients with healing disorders, such as diabetics (Vermeulen et al, 2005; Waldron and Zimmerman-Pope, 2007). Previous studies observed that HBOT increases tissue tolerance to ischemia and reduces their effects, improves tissue microcirculation by reducing platelet aggregation and increasing plasma capacity to transport oxygen to the capillary extremities. It has antioedematose, antimicrobial and antibacterial effects (Kiumehr et al, 2005; Millar et al, 2015). Pulmonary toxicity can occur after prolonged exposure to hyperbaric oxygen (Olszewer, 2008, Lima et al, 2014) For all these reasons, the importance of establishing safe protocols for patients undergoing hyperbaric therapy is highlighted (Kiumehr et al, 2005). We built a hyperbaric chamber in order to evaluate the effects of hyperbaric oxygen therapy on the wound healing in BALB/C mice and tested different HBOT exposure times

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION