Abstract
The reaction of oxidized Rhus vernicifera laccase and H 2O 2 leads specifically to the formation of a stable, high affinity complex. It is characterized by an absorption band at 325 nm and is most probably formed with the type 3 site. Oxidative titrations of laccase show a different pathway from the reductive ones. This is also expressed in different Nernst coefficients observed for each half of the redox cycle (2 for reduction, 1 for oxidation). Oxidation of the type 3 site by H 2O 2 proceeds in a bimolecular reaction, whereas type 1 is oxidized in an indirect pathway.
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