Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol.

Tayana Silva De Carvalho,Anderson Manoel Herculano,Nayara Kauffmann,Adelaide Passos,Karen Renata Matos Herculano Oliveira,Alódia Brasil,Sávio Lima-Bastos,Mateus Santos-Silva,Waldo Lucas Luz,Patrick Bruno Cardoso,Evander De Jesus Oliveira Batista,Nadyme Assad,Amauri Gouveia,Carlomagno Pacheco Bahia,Suellen Moraes

doi:10.1155/2019/8419810

Tayana Silva De Carvalho, Anderson Manoel Herculano + Show 13 more

Open Access

https://doi.org/10.1155/2019/8419810

Copy DOI

Abstract

Anxiety is a common symptom associated with high caffeine intake. Although the neurochemical mechanisms of caffeine-induced anxiety remain unclear, there are some evidences suggesting participation of oxidative stress. Based on these evidences, the current study is aimed at evaluating the possible protective effect of alpha-tocopherol (TPH) against anxiety-like behavior induced by caffeine (CAF) in zebrafish. Adult animals were treated with CAF (100 mg/kg) or TPH (1 mg/kg)+CAF before behavioral and biochemical evaluations. Oxidative stress in the zebrafish brain was evaluated by a lipid peroxidation assay, and anxiety-like behavior was monitored using light/dark preference and novel tank diving test. Caffeine treatment evoked significant elevation of brain MDA levels in the zebrafish brain, and TPH treatment prevented this increase. Caffeine treatment also induced anxiety-like behavior, while this effect was not observed in the TPH+CAF group. Taken together, the current study suggests that TPH treatment is able to inhibit oxidative stress and anxiety-like behavior evoked by caffeine.

Highlights

Caffeine (1,3,7-trimethylxanthine) is a psychoactive drug widely consumed around the world [1]
It is widely showed that glutamate and reactive oxygen species (ROS) lead to neurobehavioral alterations [11], it remains unclear if brain oxidative stress represents a biochemical mechanism involved in anxiety-like behavior evoked by caffeine
Our results have shown that zebrafish treated with caffeine spent less time in the white compartment (Figure 1(a): control group (CTRL) = 62:24 ± 11:71%/CAF = 9:42 ± 2:50%; F = 19:5875; p < 0:01)

Summary

Introduction

Caffeine (1,3,7-trimethylxanthine) is a psychoactive drug widely consumed around the world [1]. It is widely described that low doses of caffeine exerts a positive effect on cognition, memory, and learning [3], while high doses induce mental impairment, hyperactivity, and anxiety [4,5,6]. These finds suggest that caffeine is able to promote a dual effect on the central nervous system (CNS). It is widely showed that glutamate and reactive oxygen species (ROS) lead to neurobehavioral alterations [11], it remains unclear if brain oxidative stress represents a biochemical mechanism involved in anxiety-like behavior evoked by caffeine

Methods

Results

Conclusion