Oxidative stress induces a temporary initial decrease of HSP70 content in EL4 lymphoma cells

Abstract

Event Abstract Back to Event Oxidative stress induces a temporary initial decrease of HSP70 content in EL4 lymphoma cells Olga Shustova1*, Anna Boyko1, Anna Klinkova1, NATALIA TROYANOVA1, Elena Kovalenko1 and Alexander Sapozhnikov1 1 M.M. Shemyakin and Yu.A. Ovchinnikov Institute of bioorganic chemistry of the Russian Academy of Sciences, Russia HSP70 participate in intracellular processes maintaining cell homeostasis. On the other hand, under influence of various stress factors HSP70 are released from many types of cells into extracellular milieu. It was shown that extracellular HSP70 possess essential immunomodulatory properties. Besides, it was demonstrated that serum level of extracellular HSP70 circulating in organism was increased in patients with some types of diseases. Previously we demonstrated stress-induced HSP70 release in EL4 mouse lymphoma cell culture. In the present work we analyzed stress-induced alterations of intracellular level of HSP70 in the same model of EL4 cell culture. The cells were exposed to oxidative stress by using H2O2. Intracellular content of HSP70 was measured by flow cytofluorimetry. The results showed that EL4 cell reaction to oxidative stress had an initial temporary stage with a significant fall of intracellular HSP70 content. This stage was followed by expected increase of the level of intracellular HSP70. Initial decrease of intracellular HSP70 content in this model was registered also by using Western-blot analysis. We suppose that the phenomenon of temporary fall of intracellular HSP70 at the first stage of cell stress response is connected with the protein release to extracellular space. This supposition allows us to consider lymphoid cell populations as a source of soluble extracellular pool of HSP70 in serum of peripheral blood of stressed organisms. This work was partly supported by RFBR, grants # 12-04-31578 and # 12-04-32065. Acknowledgements This work was partly supported by RFBR, grants # 12-04-31578 and # 12-04-32065. Keywords: Oxidative Stress, Lymphoma, HSP70 Heat-Shock Proteins, protein release, Stress responses Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Shustova O, Boyko A, Klinkova A, TROYANOVA N, Kovalenko E and Sapozhnikov A (2013). Oxidative stress induces a temporary initial decrease of HSP70 content in EL4 lymphoma cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00167 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Olga Shustova, M.M. Shemyakin and Yu.A. Ovchinnikov Institute of bioorganic chemistry of the Russian Academy of Sciences, Moscow, Russia, olga_shustova@list.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga Shustova Anna Boyko Anna Klinkova NATALIA TROYANOVA Elena Kovalenko Alexander Sapozhnikov Google Olga Shustova Anna Boyko Anna Klinkova NATALIA TROYANOVA Elena Kovalenko Alexander Sapozhnikov Google Scholar Olga Shustova Anna Boyko Anna Klinkova NATALIA TROYANOVA Elena Kovalenko Alexander Sapozhnikov PubMed Olga Shustova Anna Boyko Anna Klinkova NATALIA TROYANOVA Elena Kovalenko Alexander Sapozhnikov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.