Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells.

Martin Kello,Klaudia Petrova,Peter Takac,Tomas Kuruc,Jan Mojzis,Peter Kubatka

doi:10.3390/biom10010139

Abstract

In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferation, migration and invasion of tumour inhibition, and sensitization of tumours to radiotherapy and chemotherapy. In this study, the ability of clove buds extract (CBE) to induce oxidative stress, DNA damage, and stress/survival/apoptotic pathways modulation were analysed in MCF-7 cells. We demonstrated that CBE treatment induced intrinsic caspase-dependent cell death associated with increased oxidative stress mediated by oxygen and nitrogen radicals. We showed also the CBE-mediated release of mitochondrial pro-apoptotic factors, signalling of oxidative stress-mediated DNA damage with modulation of cell antioxidant SOD (superoxide dismutase) system, and modulation activity of the Akt, p38 MAPK, JNK and Erk 1/2 pathways.

Highlights

Clove, a spice obtained from the dried flower of the tree Syzygium aromaticum L., has been used in traditional medicine for centuries as an antiseptic, analgesic, or in dental care [1]
clove buds extract (CBE) treatment with both c2oAn)c.eMntorraetoiovnesr,incydtuocehdrosmigencifircealenatsreelaenadseinocfrceyatsoecdheroxmpreescssiononofafStmera2c4/DhIAofBiLnOcu(bFaitgiuorne(2FBig)uarfete2rA4)8
Because MCF-7 cells do not express caspase-3 [31], we suggest that poly (ADP-ribose) polymerase (PARP) cleavage can be the result of CBE-induced caspase-7 activation

Summary

Introduction

A spice obtained from the dried flower of the tree Syzygium aromaticum L., has been used in traditional medicine for centuries as an antiseptic, analgesic, or in dental care [1]. Several studies document a broad range of biological effects of clove including antibacterial [2,3], antifungal [4], antimutagenic [5], antihistaminic [6], antiinflammatory [7], or antioxidant [8]. The antiproliferative activity of clove essential oil or eugenol, its main component, has been described [9,10]. The study of Liu et al [11] showed the antiproliferative effect of clove extract against different types of cancer cells including breast, liver, ovarian or cervical. The anticancer effect of clove buds extract (CBE) was documented in a model of chemically-induced mammary carcinogenesis [12]. In particular in vitro experiments, a significant pro-apoptotic effect of CBE has been found associated with cell cycle arrest in S phase, mitochondrial dysfunction, and apoptosis induction

Methods

Results

Conclusion