Oxidative Stress Induced by Carbonate Salinization in Triticale Seedlings

Abstract

The effect of short-term salt stress induced by carbonate salinization on the indicators of oxidative stress, the activity of antioxidant enzymes, and the content of some metabolites in winter triticale seedlings were studied. The experiment was carried out on 7-day-old plants after 12, 24, 48, 72, and 96 h of exposure to 120 mM sodium carbonate solution. The content of malonic dialdehyde, hydrogen peroxide, and the activity of enzymes (catalase, guaiacol peroxidase, and ascorbate peroxidase) were determined. It was shown that carbonate salinization causes significant changes in all mentioned parameters. Thus, more than a threefold increase in the content of hydrogen peroxide was noted in triticale shoots already after 12 h of the experiment. Intensified lipid peroxidation was also observed in the shoots during the first days of salinization. Less significant increase in both indicators was recorded in the roots. An increased activity of the studied enzymes (ascorbate peroxidase, guaiacol peroxidase, and catalase) was noted in the shoots. The analysis of the results showed that the specificity of carbonate salinization can be attributed to more intense accumulation of hydrogen peroxide in the shoots compared with the roots and a decrease in MDA content in triticale shoots by 48 h of exposure.