Oxidative Stress in Streptozotocin-Induced Rats and The Role of Bajakah (Uncaria Sp.) as Antioxidants

Abstract

Hyperglycemia in diabetes mellitus (DM) increases the production of reactive oxygen species (ROS). That condition causes a decrease in antioxidant defense activity in the body so that the body cannot fight free radicals and triggers diabetic macroangiopathy complications. One source of natural antioxidants is the Bajakah stem (Uncaria sp.). which has antioxidant and antihyperglycemic effects. so it is expected to inhibit oxidative damage in DM. This study aimed to determine the effect of the extract of bajakah stem on antihyperglycemic activity. the antioxidant enzyme activity of SOD. and MDA levels in streptozotocin-induced rats. This study used an experimental method consisting of 5 treatments: two control groups (NC. PC) and three treatment groups (P1. P2. P3). and five replications for 21 days. Antioxidant activity was measured by scavenging the activity of hydroxyl radicals. Furthermore. testing of antihyperglycemic and antioxidant activity using test animals (rats). divided into three doses. namely 100. 200. and 400 mg/kg BW. This study used one-way ANOVA and Duncan's mean difference test. Results showed that the ethanol extract of the bajakah stem (Uncaria sp.) contained alkaloids. flavonoids. tannins. saponins. triterpenoids. and steroids. The antioxidant activity test of the pirated extract obtained IC50 55.646 ppm. and a dose of 100 mg/kg BW had the best activity as antihyperglycemic. increased SOD enzyme activity. and decreased MDA levels