Oxidative stress in MCF-7 cells is involved in the effects of retinoic acid-induced activation of protein kinase C-δ on insulin-like growth factor-I secretion and synthesis

Abstract

It is known that all- trans retinoic acid (RA) is a useful therapeutic anticancer agent in breast cancer that acts by inducing apoptosis and growth inhibition. Insulin-like growth factor-I (IGF-I) is also known to be a growth hormone that plays an important role in cell proliferation and apoptosis. We examined the relationships between RA-induced protein kinase C (PKC)-δ, the secretion and synthesis of IGF-I, and oxidative stress. RA at 10 −8 M and 10 −7 M increased PKC-δ phosphorylation (the ratio of phosphorylated to total PKC-δ) ( p < 0.05) and decreased the secretion and synthesis of IGF-I ( p < 0.05) compared to control, with the effects peaking for treatment with 10 −7 M RA for 72 h. The silencing of PKC-δ prevented the RA-induced inhibition of the secretion and synthesis of IGF-I and cell viability ( p < 0.05). Application of 10 −7 M RA for 72 h increased the level of thiobarbituric-acid-reactive substances and the expression of inducible nitric oxide synthase relative to control ( p < 0.05). These increases were blocked by suppressing PKC-δ and by pretreatment with the antioxidants glutathione and diphenyleneiodonium ( p < 0.05). These antioxidants also reversed the RA-induced inhibition of the secretion and synthesis of IGF-I and cell viability to control levels ( p < 0.05). The effects of suppressing IGF-I demonstrate that IGF-I plays a critical role in the RA-induced inhibition of the cell viability. These results indicate that the anticancer effects of RA are mediated by inhibition of the secretion and synthesis of IGF-I, and involve a PKC-δ-dependent mechanism, and they provide evidence of an interaction between PKC-δ and reactive oxygen species.