Oxidative Stress Evaluation in Overweight Subjects with or without Metabolic Syndrome

Abstract

This cross‐sectional study was performed with 123 subjects (50 controls and 73 overweight). The control group included 50 healthy individuals (body mass index ‐ BMI between 20 and 24.9 kg/m2) and without MetS. Overweight group consisted of 73 subjects (BMI between 25 and 29.9 kg/m2). Overweight subjects were divided into two groups: with MetS (29 subjects) and without MetS (44 subjects). Control group and overweight subjects without MetS showed no differences in oxidative stress parameters and total antioxidant capacity (TRAP). Overweight subjects with MetS had higher hydroperoxide concentrations measured by chemiluminescence compared to the control group (p < 0.05), higher hydroperoxide and hydrogen peroxide concentrations determined by ferrous oxidation‐xylenol orange assay compared to overweight subjects without MetS (p < 0.001), and higher advanced oxidation protein products (AOPP) concentrations (p < 0.001) compared to the other groups. AOPP was directly correlated with uric acid concentrations. Overweight subjects with metabolic syndrome had lower TRAP concentrations compared to the control group (p < 0.001), when TRAP was corrected by uric acid concentration. In conclusion, this study showed that the presence of MetS is the main determinant for the redox imbalance, characterized by increased plasma oxidation and reduced antioxidant capacity, verified in overweight subjects.