Oxidative stress biomarkers in long-term participants in clean-up work after the Hebei Spirit oil spill

Abstract

BackgroundThe oil tanker Hebei Spirit spilled 12,547kL of oil near the western coastline of Korea on December 7, 2007. We aimed to investigate the relationship between oil spill exposure and oxidative stress in residents living near the affected area. MethodsStudy subjects were 671 residents who participated in a health examination between February and September 2009. As surrogates for oil spill exposure, we used the total duration of clean-up work and levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs), 1-hydroxypyrene (1-OHP) and 2-naphthol (2-NAPH). Oxidative stress was measured using urinary levels of malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG), indicators of lipid peroxidation and oxidative DNA damage, respectively. ResultsLevels of oxidative stress biomarkers were significantly increased with longer involvement in clean-up work over one year after the Hebei Spirit oil spill (MDA, p-trend<0.0001; 8-OHdG, p-trend<0.0001). As more time elapsed since the last involvement in clean-up, the total duration of clean-up work participation and levels of PAH metabolites (1-OHP and 2-NAPH), as well as levels of the oxidative stress biomarkers (MDA and 8-OHdG) decreased further. The level of 1-OHP had a significant positive correlation with the total duration of clean-up work involvement, with a higher level found in those who participated in clean-up for >100days. Increasing levels of 1-OHP were significantly associated with increased MDA and 8-OHdG after adjusting for covariates, while the strength of association weakened as time passed since the last participation in clean-up work. The significance of the association was maintained for up to 12months after the last clean-up work. ConclusionsThe results suggested that oil exposure from prolonged clean-up activity likely induced oxidative stress in clean-up participants up to at least one year after the last exposure.