OXIDATIVE STRESS AND MORPHOLOGICAL ASSESSMENT OF BONE MARROW IN MONOSODIUM GLUTAMATE-TREATED RAT

Abstract

Excess consumption of monosodium glutamate (MSG) was reported to cause oxidative stress on brain, liver and renal and altered haematological parameters. Therefore, this study was aimed to investigate the effect of MSG on oxidative stress status on bone marrow of rats. Male Sprague-Dawley rats (n=24) weighing between 160-200 g were divided randomly into three groups: Control which was given distilled water (1 mg/kg), MSG 60 and MSG 120 which were given 60 mg/kg MSG and 120 mg/kg MSG, respectively. All substances were oral force fed for 28 days consecutively. At the end of the study, bone marrow cells were isolated by flushing technique for measurement of the oxidative stress status and bone marrow smear observation. Results showed that the superoxide dismutase activity and protein carbonyl level were significantly increased in MSG 120 group than to control and MSG 60 groups (p<0.05). Conversely, glutathione level had declined significantly in both MSG groups as compared to control group (p<0.05). The malondialdehyde level was not significantly affected in MSG groups than to control group. Bone marrow smear indicated no evidence of morphological alteration in all groups. In conclusion, MSG at both doses caused oxidative stress on bone marrow after 28 days of exposure.