Oxidative Stress and Hepatotoxicity Induced by Synthetic Pyrethroids-Organophosphate Insecticides Mixture in Rat

Abstract

The effects of organophosphorus [fenitrothion (FNT)] and pyrethroid (lambda-cyhalothrin (LC)] pesticides mixture on the antioxidant defense system and liver function enzymes in rat liver were assessed. Various concentrations of FNT + LC were incubated with liver homogenate at 37°C for various incubation times. Results showed that FNT plus LC caused a significant concentration-dependent and time-related inhibition of glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities, and reduction of glutathione (GSH) content. The depletion of GSH might indicate that reactive oxygen species could be involved in the toxic effects of FNT plus LC. In addition, a significant inhibition in transaminases (AST, ALT) and phosphatases (AcP, AlP) activities was observed. On the other hand, FNT + LC increased the activity of lactate dehydrogenase (LDH) and thiobarbituric acid reactive substances (TBARS) levels. It can be concluded that such biomarkers may be used in assessing adverse/toxic effects of pesticides as environmental stressors.