Abstract
Green nanoparticles represent a revolution in bionanotechnology, providing opportunities to fight life-threatening diseases, such as cancer, with less risk to the environment and to human health. Here, for the first time, we systematically investigated the anticancer activity and possible mechanism of novel silver nanoparticles (N-SNPs) synthesized by Nostoc Bahar M against the MCF-7 breast cancer cells, HCT-116 colorectal adenocarcinoma cells, and HepG2 liver cancer cells, using cell viability assays, morphological characterization with inverted light and transmission electron microscopy, antioxidants and enzymes (glutathione peroxidase (GPx), glutathione (GSH), adenosine triphosphatase (ATPase), and lactate dehydrogenase (LDH)), and western blotting (protein kinase B (Akt), phosphorylated-Akt (p-Akt), mammalian target of rapamycin (mTOR), B-cell lymphoma 2 (Bcl-2), tumor suppressor (p53), and caspase 3). N-SNPs decreased the viability of MCF-7, HCT-116, and HepG2 cells, with half-maximal inhibitory concentrations of 54, 56, and 80 µg/mL, respectively. They also significantly increased LDH leakage, enhanced oxidative stress via effects on antioxidative markers, and caused metabolic stress by significantly decreasing ATPase levels. N-SNPs caused extensive ultrastructural alterations in cell and nuclear structures, as well as in various organelles. Furthermore, N-SNPs triggered apoptosis via the activation of caspase 3 and p53, and suppressed the mTOR signaling pathway via downregulating apoptosis-evading proteins in MCF-7, HCT-116, and HepG2 cells. Ultrastructural analysis, together with biochemical and molecular analyses, revealed that N-SNPs enhanced apoptosis via the induction of oxidative stress and/or through direct interactions with cellular structures in all tested cells. The cytotoxicity of Nostoc-mediated SNPs represents a new strategy for cancer treatment via targeting various cell death pathways. However, the potential of N-SNPs to be usable and biocompatible anticancer drug will depend on their toxicity against normal cells.
Highlights
Cancer has a major impact on human life today, owing to enormous changes in lifestyle, and it is the second leading cause of death worldwide [1]
The results showed that novel silver nanoparticles (N-SNPs) significantly inhibited the proliferation of all tested cells in a dose-dependent manner as compared with untreated cells (Figure 1A)
We conclusively demonstrated the cytotoxicity of novel N-SNPs against MCF-7, HepG2, and HCT-116 cells by various experiments, including cell viability assays, inverted light microscopy and Transmission Electron Microscopy (TEM), antioxidative marker and enzyme analysis, and western blotting assays
Summary
Cancer has a major impact on human life today, owing to enormous changes in lifestyle, and it is the second leading cause of death worldwide [1]. Cancer cells have the ability to evade apoptosis via controlling the expression of certain genes; that is, the upregulation of the expression of genes that favor survival and proliferation, and downregulation of genes that are responsible for the regulation of cell death pathways [3]. Conventional anticancer therapies, such as chemotherapeutic drugs, radiation, and surgery, are successful to some extent, but their use is limited by serious adverse effects and poor diagnosis, and by the potential for cancer cells to develop resistance to chemotherapeutic drugs [4]. Nanotechnology has been used to develop next-generation platforms for cancer diagnosis, therapy, and management [5,6,7]
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