Abstract

The effect of red pepper supercritical fluid extracts (SFE) on the oxidative stability of extra-virgin olive oil was evaluated using accelerated stability tests [Rancimat and differential scanning calorimetry (DSC) methods] and by measuring the changes in the levels of polyunsaturated fatty acid primary and secondary oxidation products during storage under ambient conditions. SFE were produced according to a central composite rotatable design, at a constant temperature (40 °C), different pressures (15–23 MPa) and superficial velocities (0.04–0.08 cm/s). The results showed that the red pepper extracts produced at low extraction pressure and superficial velocity (e.g. 16.2 MPa and 0.046 cm/s) containing low/intermediate capsaicinoid levels did not affect olive oil stability. The extracts produced at higher pressure showed a slight pro-oxidant activity. The K232 and K270 values always fell within the limit set by the European legislation for the quality characteristics of olive oil containing no additives. Evaluation of oxidative stability using DSC was found to be a useful methodology, which demands smaller oil samples and shorter times in comparison with the methodology using the Rancimat apparatus. Red pepper SFE obtained at low extraction pressures can be used in order to produce stable flavoured olive oils.

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