Oxidative responses in small juveniles of Colossoma macropomum anesthetized and sedated with Ocimum gratissimum L. essential oil.

Abstract

This study evaluated the use of essential oil of Ocimum gratissimum (EOOG) for anesthesia and in transport of Colossoma macropomum. Experiment 1, Test 1, anesthesia induction and recovery times were determined using different EOOG concentrations (0, 20, 50, 100, 200, 300mg L-1), with two size classes: Juveniles I (0.86g) and Juveniles II (11.46g) (independent tests in a completely randomized design). Based on the results of Test 1, in Test 2 Juveniles II were exposed to EOOG concentrations: 0, 20, 100mg L-1. Tissue samples were collected immediately after induction and 1h post-recovery, to assess oxidative status variables. Experiment 2, Juveniles I (0.91g) and Juveniles II (14.76g) were submitted to transport in water with different concentrations of EOOG (0, 5, 10mg L-1) (independent tests in a completely randomized design). The effects on oxidative status variables were evaluated. Concentrations between 50 and 200mg L-1 EOOG can be indicated for Juveniles I, while concentrations between 50 and 100mg L-1 EOOG for Juveniles II. The concentration of 100mg L-1 EOOG was able to prevent oxidative damage in the liver. In Experiment 2, the concentrations of 5 and 10mg L-1 EOOG added to the transport water caused sedation for both studied size classes of juveniles and did not cause oscillations in water quality variables nor any mortality. The concentration of 10mg L-1 EOOG improved the oxidative status. It can be concluded that EOOG can be used for anesthesia and transport of C. macropomum.