Abstract
IT has been presumed that in anaerobic respiration, such as nitrate1 or sulphate2 respiration, formation of some high-energy phosphate is coupled with the process in a similar way to oxidative phosphorylation in aerobic respiration. We have reported3 that, in the whole cell system of Pseudomonas denitrificans, radioactive inorganic phosphate (32Pi) is incorporated into labile organic phosphates coupled with the denitrification reaction where nitrite plays a part as a terminal electron acceptor in the anaerobic condition. In this communication more direct evidence that phosphorylation is coupled with aerobic (oxygen) and anaerobic (nitrate) respiration in the cell-free system of this bacteria is described.
Published Version
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