Oxidative metabolism altered by plant growth regulators in atemoia seeds (Annona x atemoya Mabb.) ‘Thompson’

Ana Paula Yuki Hata,Breno Kennedy Lima Bezerra,Gisela Ferreira,Marília Caixeta Sousa,Camila Kissmann

doi:10.1590/0100-29452021114

Ana Paula Yuki Hata, Breno Kennedy Lima Bezerra + Show 3 more

Open Access

https://doi.org/10.1590/0100-29452021114

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Abstract

Abstract The objective of this work was to validate the enzymatic activity in Annona x atemoyaseeds submitted to treatments with plant growth regulators belonging to the group of gibberellins and cytokinins for overcoming dormancy during the germination process. Initially, the water acquisition curve was determined, where two points of phase II (72 and 144 hours) were determined to evaluate the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes in atemoya seeds after the application of two plant growth regulators. Treatments with GA resulted in higher activity of SOD enzyme, which may be favored the higher germination percentage and speed in comparison to control treatment. In cases in which SOD enzyme presented high activity, other enzymes showed the opposite, which indicates the predominantly production of superoxides during this period. The POD enzyme also has activity in seeds treated with gibberellin, which suggests interaction of ROS with GA in the process of dormancy overcoming. Treatments with cytokinin and cytokinin + gibberellin resulted in germination percentage similar to control and no activity pattern of SOD, POD and CAT enzymes that could be correlated with germination metabolism was observed.

Highlights

The seed germination process, which begins with seed hydration and metabolism reactivation and results in the protrusion of the primary root (BEWLEY et al, 2013),is influenced by several intrinsic and extrinsic factors and included in the first group reactive are the oxygen species (ROS) (GOMES; GARCIA, 2013)
Atemoia seeds used to determine the initial water acquisition curve and for experiment 1 had initial water content of 31.5% ± 2.3% and seeds of lot 2, which were stored for five months in paper bags in refrigerator that were used for experiment 2 had water content of 7% ± 0.9% at the time of conduction of experiments
Water acquisition curves were determined for seeds treated with plant growth regulators

Summary

Introduction

The seed germination process, which begins with seed hydration and metabolism reactivation and results in the protrusion of the primary root (BEWLEY et al, 2013),is influenced by several intrinsic and extrinsic factors and included in the first group reactive are the oxygen species (ROS) (GOMES; GARCIA, 2013). The aim of this work was to evaluate the effect of plant regulators (gibberellins and cytokinins) on the oxidative metabolism through the activity of superoxide dismutase, peroxidase and catalase enzymes on Annona x atemoya Mabb seeds.

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