Abstract
Oxidative Decay of DNA
Highlights
Together with the fact that radically different techniques have demonstrated similar degrees and patterns of induced DNA damage, these results suggest that the problem of artifactual noise has been tamed, if not eliminated
What is encouraging about recent results is the growing congruence between studies using different approaches
Until the last 2 years, it had become almost accepted wisdom that the role of the superoxide anion radical (O2.) in DNA oxidation was its ability to reduce ferric iron (Fe3ϩ) to ferrous iron (Fe2ϩ); Fe2ϩ catalyzes the formation of the hydroxyl radical 1⁄7OH
Summary
Methods for Measuring Oxidative DNA Damage The steady-state amount of DNA oxidation appears to be massive, with oxidative adducts occurring at a frequency that is 1 or more orders of magnitude higher than non-oxidative adducts [1, 7] Despite their abundance, oxidative DNA adducts exist in a large background [105–106] of unaltered nucleotides, which may be prone to oxidation during sample preparation and analysis. A technique for estimating the rate of oxo8dG formation, which does not require the isolation of DNA and its associated problems, is the measurement of its repair products excreted into urine or tissue culture medium [3, 28, 29]. What is encouraging about recent results is the growing congruence between studies using different approaches
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