Oxidative damage to nucleic acids and benzo(a)pyrene-7,8-diol-9,10-epoxide-DNA adducts and chromosomal aberration in children with psoriasis repeatedly exposed to crude coal tar ointment and UV radiation.

Lenka Borska,Jan Krejsek,Zdenek Fiala,Ctirad Andrys,Marcela Chmelarova,Vladimir Palicka,Kvetoslava Hamakova,Jan Kremlacek

doi:10.1155/2014/302528

Abstract

The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n=19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score).

Highlights

Oxidative stress, characterized by a high degree of reactive oxygen species (ROS), is known to induce damage to cellular components including strand breaks and base modifications in nucleic acids [1]
For analysis of selected polycyclic aromatic hydrocarbons (PAHs), we used a pooled sample of crude coal tar (CCT)
As resulted from analysis of CCT (Table 2), one PAH is sorted into the group of proven carcinogens, one PAH in the group of probably carcinogens, and six PAHs into the group of possibly carcinogens

Summary

Introduction

Oxidative stress, characterized by a high degree of reactive oxygen species (ROS), is known to induce damage to cellular components including strand breaks and base modifications in nucleic acids [1]. Oxidative damage to nucleic acids has been found to be associated with a variety of diseases including cancer and aging, but the precise mechanisms are still remaining to be elucidated [2, 3]. Psoriasis is a multifactorial relapsing and remitting inflammatory skin disease. The prevalence of psoriasis ranged from 1.4% to 3.3% in North America and Europe [4]. The disease represents about 4% of all dermatoses which originally manifested at 16 years of age or younger, often as Oxidative Medicine and Cellular Longevity plaque psoriasis [5, 6]. Psoriasis can adversely affect quality of life of children [7]

Methods

Results

Discussion

Conclusion