Oxidative damage of nanomaterial cells in gastrointestinal smooth muscle movement

Abstract

If gastrointestinal smooth muscle suffers from oxidative damage, the risk of canceration is very high. Gold nanostars/collagen (AuNSs/Col) solutions of 0, 0.05, 0.10, 0.15 and 0.20 mg/ml were prepared by dissolving the new AuNSs in rat tail collagen solution. Live/dead staining was used to observe the survival rate and proliferation rate of cells under a fluorescence microscope, and the superoxide dismutase activity and malondialdehyde content were calculated according to the values. The glutathione peroxidase (GSH-Px) reagent was added to the two groups, and the optical density values of each tube were measured by using an enzyme scale instrument. The activity of GSH-Px was calculated according to the value, and the reactive oxygen species level was expressed by fluorescence intensity. Live/dead staining showed that the lower the concentration of AuNSs/Col, the higher the survival rate of GES-1 cells. The 0.1 mg/ml AuNSs/Col group had no toxicity to GES-1 and could further promote the proliferation of GES-1. The 0.1 mg/ml AuNSs/Col composite had the highest proliferation activity to GES-1. The higher the concentration of AuNSs/Col nanocomposites is, the more serious the oxidative damage of GES-1 cells is.