Oxidative damage of kidney, heart, lung, and spleen measured by oxidized heme proteins in tissue slices

Abstract

Oxidative damage to rat Kidney, heart, lung, and spleen was measured by the formation of oxidized heme proteins (OHP) in tissue slices. Kidney, heart, lung, and spleen slices were incubated in oxygenated KRP-glucose buffer at 31 o C with and without the presence of prooxidants. The absorbance spectra (500-640 nm) of heme proteins in the fresh and oxidized tissue slices were analyzed, and the concentrations of OHP, such as methemoglobin, ferrylhemoglobin, hemichrome, oxidized mitochondrial, and microsomal cytochromes were determined with a heme protein spectra analysis program