Abstract
The purpose of this study was to investigate the effect of local ultraviolet radiation on the content of lipid peroxidation products, lipofuscin, and the functioning of antioxidant enzymes in the skin of guinea pigs. Materials and methods. The studies were performed on 24 albino guinea pigs exposed to a single local ultraviolet radiation. The control group was made up of intact animals. The content of primary (diene conjugates) and secondary (TAC-active products) lipid peroxidation products, the activity of antioxidant enzymes (superoxide dismutase and catalase), and the content of lipofuscin were assessed in the skin in 2 hours, 4 hours, and on the 3rd and 8th day following the UV irradiation. Results. Under the influence of local ultraviolet radiation, all guinea pigs develop erythema (its peak is observed in 4 hours, and on the 3rd day), which disappears on the 8th day. There is a sharp enhancement of lipid peroxidation processes due to the accumulation of lipid peroxidation primary and secondary products in the skin of guinea pigs in all periods of the experiment. In parallel with the increase in lipid peroxidation products, there is a decrease in the activity of antioxidant enzymes (catalase and superoxide dismutase) in the focus of radiation. The content of lipofuscin in the skin progressively increases throughout the study period. Conclusions. The early erythema period following the local ultraviolet irradiation in the skin of guinea pigs, is characterized by increased content of diene conjugates and TBA-active products (in 2, 4 hours, on the 3rd day); decreased catalase activity and increased lipofuscin concentration (on the 3rd day after irradiation). The early post-erythema period (8th day) following the local ultraviolet irradiation is characterized by the accumulation of diene conjugates, TBA-active products and lipofuscin against the background of a decrease in the activity of catalase and superoxide dismutase in the skin of guinea pigs.
Highlights
The purpose of this study was to investigate the effect of local ultraviolet radiation on the content
The studies were performed on 24 albino guinea pigs exposed to a single local ultraviolet radiation
the content of lipofuscin were assessed in the skin in 2 hours
Summary
Цель исследования – изучить влияние локального ультрафиолетового облучения на содержание продуктов перекисного окисления липидов, липофусцина, активность антиоксидантных ферментов в коже морских свинок. Через 2 часа, 4 часа, на 3-и и 8-е сутки после облучения в коже определяли содержание первичных (диеновых конъюгатов) и вторичных (ТБК-активных продуктов) продуктов перекисного окисления липидов, активность антиоксидантных ферментов (супероксиддисмутазы и каталазы), содержание липофусцина. 1. В ранний эритемный период после локального ультрафиолетового облучения в коже морских свинок наблюдается повышение содержания диеновых конъюгатов и ТБК-активных продуктов (через 2, 4 часа, 3-и сутки); снижение активности каталазы и увеличение концентрации липофусцина (на 3-и сутки после облучения). 2. Ранний постэритемный период (8-е сутки) после локального ультрафиолетового облучения характеризуется накоплением диеновых конъюгатов, ТБКактивных продуктов и липофусцина на фоне снижения активности каталазы и супероксиддисмутазы в коже морских свинок. С окислительным стрессом тесно связано возрастное накопление пигмента липофусцина, что становится причиной накопления поврежденных митохондрий и других органелл, и, как следствие, клеточной дисфункции постмитотических клеток, дисфункции органов и старения [3,4]
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