Abstract

Dissimilation of glucose is brought about by bacteria of the genus Aerobacter and among the products of fermentation are acetyl-methyl-carbinol and its reduction product, 2, 3-butylene glycol. It has been suggested that these substances form a reversible oxidation-reduction system. In this case the methylene blue may replace the acetyl-methyl-carbinol as a hydrogen acceptor providing its redox potential lies above that of the carbinol-glycol system. The substances acting as hydrogen donators include the reduced constituents of all the reversible systems present in fermentations whose electrode values lie below that of methylene blue. In 2, 3-butylene glycol fermentations, lactic acid might be expected to donate hydrogen and form pyruvic acid. If 2, 3-butylene glycol is involved in a reversible system whose electrode potential value is below that of methylene blue, the addition of methylene blue to the fermenting mixture will reduce the concentration of 2, 3-butylene glycol and increase concentration of ac...

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