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Abstract
Dithionite reduced azoferredoxin and molybdoferredoxin from Clostridium pasteurianum W5 were oxidatively titrated with various electron acceptors. The AzoFd gave up 0.87 electrons per AzoFd monomer (27,500 mol. wt.). The oxidation reduction potential of AzoFd, determined by equilibrium with redox dyes, was −0.240 V. Dithionite reduced MoFd gave up 3.6 electrons per MoFd tetramer (220,000 mol. wt.). The oxidation reduction potential for MoFd was −0.070 V. Because the potential of this MoFd half cell is so positive, the electrons removed during this oxidation may not be those that reduce dinitrogen.
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