Abstract
Saturation-recovery electron paramagnetic resonance and differential scanning calorimetry were used to determine the cholesterol content at which pure cholesterol bilayer domains (CBDs) and cholesterol crystals begin to form in 1-palmitoyl-2-arachidonoylphosphochatidyline (PAPC) membranes. To preserve compositional homogeneity of the membrane suspension at a high cholesterol content, multilamellar liposome suspensions were prepared using the rapid solvent exchange method. The cholesterol content in membranes was changed from 0 mol% to 66 mol%.
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