Abstract
Nitrite is oxidized to nitrate by high concentrations of beef-liver catalase and byAspergillus flavus hyphal extracts. From 80 to 90 % of the nitrite-nitrogen is recovered as nitrate. The fungal enzyme is soluble and non-cytochrome-linked. It is distinct from the fungal catalase and apparently acts peroxidatively. Hydroxylamine is metabolized by high concentrations of catalase, and about half of the hydroxylamine which disappears is recovered as nitrate. Horseradish peroxidase catalyzes the formation of nitrate in the presence of hydroxylamine, apparently by oxidizing a product of the spontaneous decomposition of the nitrogen compound.A. flavus extracts do not oxidize hydroxylamine.
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