Abstract
Microbial oxidation and assimilation of n-tetradecane by two citric acid-producingCandida sp. (a wild typeCandida sp. KSH 21 and mutant from this strain No. 337) were studied. A monoterminal oxidation of n-tetradecane can be observed due to the isolation of 1-tetradecanol and myristic acid. Among the fatty acids detected from the culture fluid and in the cells the even-numbered dominate. No fatty acids with an odd number of carbon atoms could be isolated in the cells. A direct desaturation of myristic acid in the cells can be demonstrated by the presence of tetradecenoic acid. Beside monoterminal oxidation, diterminal oxidation can be observed by the appearance of tetradecanedioic acid. Dioic acids with an odd number of carbons can also be identified. Furthermore very many short chain dioic acids could be found in contrast to cultures grown on glucose and to other noncitric acid-producing species ofCandida. Substances which are supposed to be diols (1,14-tetradecanediol etc.) could be isolated in very small concentration from fermenter culture fluids of both strains.
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